PVRL1 Protein (AA 31-334) (His-Avi Tag)

Catalog No. ABIN7275307

This Recombinant PVRL1 protein is expressed in HEK-293 Cells.

Quick Overview for PVRL1 Protein (AA 31-334) (His-Avi Tag) (ABIN7275307)

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-334

Purification tag / Conjugate: This PVRL1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Nectin-1/PVRL1/CD111 Protein

Sequence: Gln31-Thr334

Characteristics: Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln31-Thr334.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).,Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 uM as determined in SPR assay (Biacore T200).,Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 60.7ng/ml determined by ELISA (QC Test).,Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA.,Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Nectin-1 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRL1 (Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C) (PVRL1))

Alternative Name: Nectin-1

Background: Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.

Molecular Weight: 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.

UniProt: Q15223

Pathways: Cell-Cell Junction Organization