Lipocalin 2 Protein (LCN2) (AA 21-198) (hFc Tag)

Catalog No. ABIN7275321

This Recombinant Lipocalin 2 protein is produced in HEK-293 Cells.

Quick Overview for Lipocalin 2 Protein (LCN2) (AA 21-198) (hFc Tag) (ABIN7275321)

Target: Lipocalin 2 (LCN2)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-198

Purification tag / Conjugate: This Lipocalin 2 protein is labelled with hFc Tag.

Purpose: Human NGAL/Lipocalin-2 Protein

Sequence: Gln21-Gly198

Characteristics: Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln21-Gly198.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NGAL, hFc Tag at 1 ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 21.4ng/ml determined by ELISA.,Human NGAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human NGAL is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM MES,150 mM NaCl ( pH 6.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES,150 mM NaCl ( pH 6.5). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: Lipocalin 2 (LCN2)

Alternative Name: NGAL

Background: Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.

Molecular Weight: 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

UniProt: P80188

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis