NKG2C & CD94 (AA 98-231) (Active) protein (His-Avi Tag,DYKDDDDK Tag)

Catalog No. ABIN7275330

This Recombinant NKG2C & CD94 protein is produced in HEK-293 Cells.

Quick Overview for NKG2C & CD94 (AA 98-231) (Active) protein (His-Avi Tag,DYKDDDDK Tag) (ABIN7275330)

Target: NKG2C & CD94

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 98-231

Purification tag / Conjugate: His-Avi Tag,DYKDDDDK Tag

Purpose: Non-biotinylated Human NKG2C&CD94 Protein

Sequence: Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)

Characteristics: Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2C and Flag tag at the N-Terminus of CD94.It contains Glu98-Leu231(NKG2C) and Ser34-Ile179(CD94).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).,Human NKG2C&CD94 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NKG2C & CD94

Alternative Name: NKG2C&CD94

Background: NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.

Molecular Weight: 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.

UniProt: P26717