KLRK1 Protein (AA 78-216) (hFc-DYKDDDDK Tag)
Quick Overview for KLRK1 Protein (AA 78-216) (hFc-DYKDDDDK Tag) (ABIN7275337)
Target
See all KLRK1 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 78-216
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Purification tag / Conjugate
- This KLRK1 protein is labelled with hFc-DYKDDDDK Tag.
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Purpose
- Human NKG2D/CD314 Protein
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Sequence
- Phe78-Val216
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Characteristics
- Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc (IgG1) tag and Flag tag at the N-Terminus.It contains Phe78-Val216.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).,Immobilized Human MICB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA,Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.,The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
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Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 78-216) (Active) protein (His-Avi Tag)
KLRK1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) (Active) protein (His tag)KLRK1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 99-330) protein (mFc Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 78-216) (Active) protein (Biotin,His-Avi Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 1-232) protein (His tag)custom-made KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 1-216) protein (His tag)custom-made KLRK1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 90-232) (Active) protein (His tag)KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 90-232) (Active) protein (hFc Tag)KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 73-216) (Active) protein (Fc Tag)KLRK1 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1) (AA 99-330) protein (Fc Tag)KLRK1 Origin: Mouse Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))
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Alternative Name
- NKG2D
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Background
- NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
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Molecular Weight
- 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
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UniProt
- P26718
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Pathways
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target
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