KLRC1 Protein (AA 100-233) (His-Avi Tag)

Catalog No. ABIN7275338

Recombinant KLRC1 protein expressed in HEK-293 Cells.

Quick Overview for KLRC1 Protein (AA 100-233) (His-Avi Tag) (ABIN7275338)

Target: KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 100-233

Purification tag / Conjugate: This KLRC1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human NKG2A/CD159a Protein

Sequence: Arg100-Leu233

Characteristics: Recombinant Human NKG2A/CD159a Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKG2A, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 5.8ng/ml determined by ELISA (QC Test).,Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLRC1 (Killer Cell Lectin-Like Receptor Subfamily C, Member 1 (KLRC1))

Alternative Name: NKG2A

Background: NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 Molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.

Molecular Weight: 18.3 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.

UniProt: P26715