KLRK1 Protein (AA 78-216) (His-Avi Tag)

Catalog No. ABIN7275342

This Recombinant KLRK1 protein is produced in HEK-293 Cells.

Quick Overview for KLRK1 Protein (AA 78-216) (His-Avi Tag) (ABIN7275342)

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 78-216

Purification tag / Conjugate: This KLRK1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human NKG2D/CD314 Protein

Sequence: Phe78-Val216

Characteristics: Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with Avi tag and His tag at the N-Terminus.It contains Phe78-Val216.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).,Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).,Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.,Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.,Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.,Loaded Human ULBP-2, hFc Tag (Cat. ULB-HM202) on ProA-Biosensor can bind Human NKG2D, His Tag with an affinity constant of 1.16 μM as determined in BLI assay (Gator® Prime).,The purity of Human NKG2D is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLRK1 (Killer Cell Lectin-Like Receptor Subfamily K, Member 1 (KLRK1))

Alternative Name: NKG2D

Background: NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Molecular Weight: 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Bis-Tris PAGE result.

UniProt: P26718

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process