NCR3 Protein (AA 19-138) (His-Avi Tag)

Catalog No. ABIN7275353

Recombinant NCR3 protein expressed in HEK-293 Cells.

Quick Overview for NCR3 Protein (AA 19-138) (His-Avi Tag) (ABIN7275353)

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-138

Purification tag / Conjugate: This NCR3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human NKp30/NCR3/CD337 Protein

Sequence: Leu19-Thr138

Characteristics: Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Human NKp30, His Tag with an affinity constant of 0.292 uM as determined in SPR assay (Biacore T200).,Immobilized Human NKp30, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human B7-H6, hFc Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test).,Immobilized Human NKp30, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.,Human NKp30 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Loaded Human B7-H6, hFc Tag on ProA-Biosensor, can bind Human NKp30, His Tag with an affinity constant of 0.51 μM as determined in BLI assay (Gator).,The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Alternative Name: NKp30

Background: NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

Molecular Weight: 20 kDa. Due to glycosylation, the protein migrates to 26-40 kDa based on Bis-Tris PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process