NCR3 Protein (AA 19-138) (Biotin,His-Avi Tag)

Catalog No. ABIN7275354

This Recombinant NCR3 protein is produced in HEK-293 Cells.

Quick Overview for NCR3 Protein (AA 19-138) (Biotin,His-Avi Tag) (ABIN7275354)

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-138

Purification tag / Conjugate: This NCR3 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human NKp30/NCR3/CD337 Protein

Sequence: Leu19-Thr138

Characteristics: Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu19-Thr138.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human B7-H6, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human NKp30, His-Avi Tag with an affinity constant of 0.330 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human NKp30, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).,Biotinylated Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human NKp30 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NCR3 (Natural Cytotoxicity Triggering Receptor 3 (NCR3))

Alternative Name: NKp30

Background: NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells.

Molecular Weight: 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on Bis-Tris PAGE result.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process