SARS-CoV-2 NSP7, NSP8 (AA 1-83) (Active) protein (His tag)

Catalog No. ABIN7275372

This Recombinant SARS-CoV-2 NSP7, NSP8 protein is produced in Escherichia coli (E. coli).

Quick Overview for SARS-CoV-2 NSP7, NSP8 (AA 1-83) (Active) protein (His tag) (ABIN7275372)

Target: SARS-CoV-2 NSP7, NSP8

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 1-83

Purification tag / Conjugate: His tag

Purpose: SARS-COV-2 NSP7&NSP8 Protein

Sequence: Ser1-Gln83(NSP7) & Ala1-Gln198(NSP8)

Specificity: Uni-Prot: YP_009725303.1 (NSP7), YP_009725304.1 (NSP8)

Characteristics: SARS-COV-2 NSP7&NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7)&Ala1-Gln198(NSP8).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: SARS-COV-2 NSP7&NSP8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM Nacl, 200 mM Arginine ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 NSP7, NSP8

Alternative Name: SARS-CoV2 NSP7 & NSP8

Target Type: Viral Protein

Background: The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19.

Molecular Weight: 32.8 kDa same as Bis-Tris PAGE result.

NCBI Accession: YP_009725303, YP_009725304