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SARS-CoV-2 NSP7, NSP8 (AA 1-83) protein (His tag)

Origin: SARS Coronavirus-2 (SARS-CoV-2) Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE
Catalog No. ABIN7275372
  • Target
    SARS-CoV-2 NSP7, NSP8
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-83
    Origin
    SARS Coronavirus-2 (SARS-CoV-2)
    Source
    • 1
    Escherichia coli (E. coli)
    Purification tag / Conjugate
    His tag
    Purpose
    SARS-COV-2 NSP7 & NSP8 Protein
    Sequence
    Ser1-Gln83 (NSP7) & Ala1-Gln198 (NSP8)
    Specificity
    Uni-Prot: YP_009725303.1 (NSP7), YP_009725304.1 (NSP8)
    Characteristics
    SARS-COV-2 NSP7 & NSP8 Protein is expressed from E.coil with His tag at the C-Terminus.It contains Ser1-Gln83(NSP7) & Ala1-Gln198(NSP8).
    Purity
    > 95 % as determined by Tris-Bis PAGE
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in 20 mM Tris, 150 mM Nacl, 200 mM Arginine ( pH 8.2). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    SARS-CoV-2 NSP7, NSP8
    Alternative Name
    SARS-CoV2 NSP7 & NSP8
    Target Type
    Viral Protein
    Background
    The crystal structure of the metabolite of remdesivir (Monophosphate of GS-441524) and NSP12-NSP8-NSP7 of SARS CoV-2 virus was recently reported. The crystal structures of ADP-Ribose or AMP and NSP3 of SARS CoV-2 virus were also released, recently. The crystal structure of NSP3 of SARS CoV-2 virus as an alternative binding site of AMP or ADP-ribose to treat COVID-19.
    Molecular Weight
    32.8 kDa same as Tris-Bis PAGE result.
    NCBI Accession
    YP_009725303
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