TNFRSF4 Protein (AA 29-216) (Biotin,His-Avi Tag)

Catalog No. ABIN7275390

This Recombinant TNFRSF4 protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF4 Protein (AA 29-216) (Biotin,His-Avi Tag) (ABIN7275390)

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 29-216

Purification tag / Conjugate: This TNFRSF4 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human OX40/TNFRSF4/CD134 Protein

Sequence: Leu29-Ala216

Characteristics: Recombinant Biotinylated Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human OX40, His Tag immobilized on CM5 Chip can bind Human OX40 Ligand, hFc Tag with an affinity constant of 23.48 nM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).,Biotinylated Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human OX40 is greater than 95% as determined by SEC HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: OX40

Background: Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation, its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Molecular Weight: 23.2 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

UniProt: P43489

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints