PLAUR Protein (AA 23-305) (His tag)

Catalog No. ABIN7275402

This Recombinant PLAUR protein is produced in HEK-293 Cells.

Quick Overview for PLAUR Protein (AA 23-305) (His tag) (ABIN7275402)

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-305

Purification tag / Conjugate: This PLAUR protein is labelled with His tag.

Purpose: Cynomolgus uPAR/PLAUR Protein

Sequence: Leu23-Gly305

Characteristics: Recombinant Cynomolgus uPAR/PLAUR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Gly305.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus uPAR, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human PLAU, His Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).,Cynomolgus uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Cynomolgus uPAR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Alternative Name: uPAR

Background: The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

Molecular Weight: 32.69 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: Q9GK78

Pathways: Inositol Metabolic Process