PLAUR Protein (AA 24-298) (His-Avi Tag,Biotin)

Catalog No. ABIN7275410

Recombinant PLAUR protein expressed in HEK-293 Cells.

Quick Overview for PLAUR Protein (AA 24-298) (His-Avi Tag,Biotin) (ABIN7275410)

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-298

Purification tag / Conjugate: This PLAUR protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Mouse uPAR/PLAUR Protein

Sequence: Leu24-Gly298

Characteristics: Recombinant Biotinylated Mouse uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human PLAU, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse uPAR isoform 1, His Tag with the EC50 of 2.57μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLAUR (Plasminogen Activator, Urokinase Receptor (PLAUR))

Alternative Name: uPAR

Background: The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR.

Molecular Weight: 32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

UniProt: P35456-1

Pathways: Inositol Metabolic Process