PD-1 Protein (AA 25-167) (His-Avi Tag,Biotin)

Catalog No. ABIN7275421

This Recombinant PD-1 protein is expressed in HEK-293 Cells.

Quick Overview for PD-1 Protein (AA 25-167) (His-Avi Tag,Biotin) (ABIN7275421)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-167

Purification tag / Conjugate: This PD-1 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human PD-1/PDCD1 Protein

Sequence: Leu25-Gln167

Characteristics: Recombinant Biotinylated Human PD-1/PDCD1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu25-Gln167.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human PD-1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 14.0ng/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Molecular Weight: 18.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

UniProt: Q15116-1

Pathways: Cancer Immune Checkpoints