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PD-1 Protein (AA 25-167) (Fc Tag)

PDCD1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Catalog No. ABIN7275419
  • Target See all PD-1 (PDCD1) Proteins
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 25-167
    Origin
    • 39
    • 15
    • 6
    • 4
    • 4
    • 3
    • 1
    Human
    Source
    • 52
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    HEK-293 Cells
    Purification tag / Conjugate
    This PD-1 protein is labelled with Fc Tag.
    Purpose
    Human PD-1/PDCD1 Protein
    Sequence
    Leu25-Gln167
    Characteristics
    Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Gln167.
    Purity
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human PD-1, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 31.2ng/ml determined by ELISA. The affinity constant of 0.344 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    Buffer
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    Storage
    -20 °C,-80 °C
    Storage Comment
    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))
    Alternative Name
    PD-1 (PDCD1 Products)
    Synonyms
    CD279 Protein, PD-1 Protein, PD1 Protein, SLEB2 Protein, hPD-1 Protein, hPD-l Protein, Ly101 Protein, Pdc1 Protein, programmed cell death 1 Protein, PDCD1 Protein, Pdcd1 Protein
    Background
    Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
    Molecular Weight
    42.6 kDa. Due to glycosylation, the protein migrates to 65-72 kDa based on Tris-Bis PAGE result.
    Pathways
    Cancer Immune Checkpoints
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