PD-1 Protein (AA 25-167) (hFc Tag,Biotin)

Catalog No. ABIN7275424

This Recombinant PD-1 protein is expressed in HEK-293 Cells.

Quick Overview for PD-1 Protein (AA 25-167) (hFc Tag,Biotin) (ABIN7275424)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-167

Purification tag / Conjugate: This PD-1 protein is labelled with hFc Tag,Biotin.

Purpose: Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling)

Sequence: Leu25-Gln167

Characteristics: Recombinant Biotinylated Mouse PD-1/PDCD1 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Biotinylated Mouse PD-1, hFc Tag with an affinity constant of 3.35 uM as determined in SPR assay (Biacore T200).,Biotinylated Mouse PD-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse PD-1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.

Molecular Weight: 42.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_032824

Pathways: Cancer Immune Checkpoints