PD-L1 Protein (AA 19-238) (Fc Tag)
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- Target See all PD-L1 Proteins
- PD-L1 (CD274 (PD-L1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 19-238
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PD-L1 protein is labelled with Fc Tag.
- Purpose
- Human PD-L1/B7-H1 Protein
- Sequence
- Phe19-Arg238
- Characteristics
- Recombinant Human PD-L1/B7-H1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe19-Arg238.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. The affinity constant of 2.356 μM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD274 (PD-L1) (AA 19-238) protein (His tag)
PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His-Avi Tag,Biotin)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (mFc Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (mFc-His Tag)PD-L1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA
CD274 (PD-L1) (AA 19-238) (Active) protein (His tag)HPLC verified PD-L1 Origin: Human Host: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
CD274 (PD-L1) (AA 19-238) protein (Fc Tag)PD-L1 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-237) protein (Fc Tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag)PD-L1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD274 (PD-L1) (AA 19-238) protein (His tag,Biotin)PD-L1 Origin: Cynomolgus, Rhesus Monkey Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- PD-L1 (PD-L1 Products)
- Background
- B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
- Molecular Weight
- 52 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
- Pathways
- Cancer Immune Checkpoints
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