PDCD1LG2 Protein (AA 20-220) (His-Avi Tag)

Catalog No. ABIN7275432

This Recombinant PDCD1LG2 protein is produced in HEK-293 Cells.

Quick Overview for PDCD1LG2 Protein (AA 20-220) (His-Avi Tag) (ABIN7275432)

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-220

Purification tag / Conjugate: This PDCD1LG2 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human PD-L2/B7-DC Protein

Sequence: Leu20-Thr220

Characteristics: Recombinant Human PD-L2/B7-DC Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Thr220.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-L2, His Tag with an affinity constant of 0.296 uM as determined in SPR assay (Biacore T200).,Human PD-L2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human PD-L2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PDCD1LG2 (Programmed Cell Death 1 Ligand 2 (PDCD1LG2))

Alternative Name: PD-L2

Background: PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.

Molecular Weight: 25.3 kDa. Due to glycosylation, the protein migrates to 40-52 kDa based on Bis-Tris PAGE result.

UniProt: Q9BQ51