PD-L1 Protein (AA 19-238) (Biotin,His tag)

Catalog No. ABIN7275436

This Recombinant PD-L1 protein is expressed in HEK-293 Cells.

Quick Overview for PD-L1 Protein (AA 19-238) (Biotin,His tag) (ABIN7275436)

Target: PD-L1 (CD274 (PD-L1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 19-238

Purification tag / Conjugate: This PD-L1 protein is labelled with Biotin,His tag.

Purpose: Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling)

Sequence: Phe19-Thr238

Characteristics: Recombinant Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Thr238.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).,Biotinylated Mouse PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.

Molecular Weight: 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_068693

Pathways: Cancer Immune Checkpoints