PDGFRA Protein (AA 24-524) (His-Avi Tag,Biotin)
Quick Overview for PDGFRA Protein (AA 24-524) (His-Avi Tag,Biotin) (ABIN7275440)
Target
See all PDGFRA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 24-524
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Purification tag / Conjugate
- This PDGFRA protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human PDGF R alpha/PDGFRA Protein
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Sequence
- Gln24-Glu524
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Characteristics
- Recombinant Biotinylated Human PDGF R alpha/PDGFRA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln24-Glu524.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Platelet Derived Growth Factor Receptor alpha (PDGFRA) (AA 24-524) protein (His-Avi Tag)
PDGFRA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet Derived Growth Factor Receptor alpha (PDGFRA) (AA 24-524) protein (His tag)PDGFRA Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Platelet Derived Growth Factor Receptor alpha (PDGFRA) protein (Fc Tag)PDGFRA Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Platelet Derived Growth Factor Receptor alpha (PDGFRA) (AA 24-528) protein (His tag)PDGFRA Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Platelet Derived Growth Factor Receptor alpha (PDGFRA) (AA 25-218) protein (GST tag)PDGFRA Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor Receptor alpha (PDGFRA) (AA 24-524) protein (His tag)PDGFRA Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Platelet Derived Growth Factor Receptor alpha (PDGFRA) protein (His tag)PDGFRA Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Platelet Derived Growth Factor Receptor alpha (PDGFRA) protein (Fc Tag)PDGFRA Origin: Rat Host: HEK-293 Cells Recombinant (96.6+2.8) % as determined by SDS-PAGE
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- PDGFRA (Platelet Derived Growth Factor Receptor alpha (PDGFRA))
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Alternative Name
- PDGF R alpha
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Background
- Platelet-derived growth factor is commonly known as a mitogen. Many research data suggest a role for PDGF-beta R in the mitogenic response of mesangial cells. There are four members of PDGF family known as PDGF-A chain, PDGF-B chain, PDGF-C chain and PDGF-D chain, which in active forms are dimers. As far as two receptors PDGF-alpha R and PDGF-beta R are known to bind PDGF.
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Molecular Weight
- 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result.
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UniProt
- P16234
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Pathways
- RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Platelet-derived growth Factor Receptor Signaling
Target
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