Phospholipase D4 Protein (PLD4) (AA 52-506) (His tag)

Catalog No. ABIN7275464

Recombinant Phospholipase D4 protein expressed in HEK-293 Cells.

Quick Overview for Phospholipase D4 Protein (PLD4) (AA 52-506) (His tag) (ABIN7275464)

Target: Phospholipase D4 (PLD4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 52-506

Purification tag / Conjugate: This Phospholipase D4 protein is labelled with His tag.

Purpose: Human PLD4 Protein

Sequence: Trp52-Gly506

Characteristics: Recombinant Human PLD4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp52-Gly506.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human PLD4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human PLD4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM MES, 100 mM NaCl ( pH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 100 mM NaCl ( pH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Phospholipase D4 (PLD4)

Alternative Name: PLD4

Background: Phospholipase D4 (PLD4) is a newly identified protein expressed in microglia. the expression of PLD4 was located in macrophages in the colon cancer mesenchymal and lymph nodes as shown by immunohistochemical analysis. furthermore, its expression was associated with clinical staging of colon cancer. Then, THP-1 as a cell model induced into TAMs. PLD4 could be involved in the activation process of M1 phenotype macrophages.

Molecular Weight: 51.1 kDa. Due to glycosylation, the protein migrates to 68-75 kDa based on Bis-Tris PAGE result.

UniProt: Q96BZ4