Microseminoprotein, Prostate Associated (MSMP) (AA 37-139) (Active) protein (hFc Tag)

Catalog No. ABIN7275492

This Recombinant Microseminoprotein, Prostate Associated protein is produced in HEK-293 Cells.

Quick Overview for Microseminoprotein, Prostate Associated (MSMP) (AA 37-139) (Active) protein (hFc Tag) (ABIN7275492)

Target: Microseminoprotein, Prostate Associated (MSMP)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 37-139

Purification tag / Conjugate: hFc Tag

Purpose: Human PSMP Protein

Sequence: Lys37-Ser139

Characteristics: Recombinant Human PSMP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Lys37-Ser139.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human PSMP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human PSMP is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Microseminoprotein, Prostate Associated (MSMP)

Alternative Name: PSMP

Background: PC3-secreted microprotein (PSMP)/microseminoprotein (MSMP) is a novel chemotactic cytokine and its receptor is CCR2. PSMP was highly expressed in fibrotic/cirrhotic tissues from patients with different etiologies of liver disease and in the 3 experimental mouse models of fibrosis. Damage-associated molecular pattern molecules HMGB-1 and IL-33 induced hepatocytes to produce PSMP. PSMP promotes liver fibrosis through inflammatory macrophage infiltration, polarization and production of proinflammatory cytokines, as well as direct activation of hepatic stellate cells via its receptor CCR2.

Molecular Weight: 37.9 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

UniProt: Q1L6U9