PSMA Protein (AA 45-752) (Biotin,His tag)

Catalog No. ABIN7275495

This Recombinant PSMA protein is produced in HEK-293 Cells.

Quick Overview for PSMA Protein (AA 45-752) (Biotin,His tag) (ABIN7275495)

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 45-752

Purification tag / Conjugate: This PSMA protein is labelled with Biotin,His tag.

Purpose: Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling)

Sequence: Lys45-Ala752

Characteristics: Recombinant Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-Terminus.It contains Lys45-Ala752.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Mouse PSMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Mouse PSMA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: PSMA

Background: Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Molecular Weight: 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result.

UniProt: O35409