TNFRSF11A Protein (AA 30-212) (Fc Tag)

Catalog No. ABIN7275521

Recombinant TNFRSF11A protein expressed in HEK-293 Cells.

Quick Overview for TNFRSF11A Protein (AA 30-212) (Fc Tag) (ABIN7275521)

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-212

Purification tag / Conjugate: This TNFRSF11A protein is labelled with Fc Tag.

Purpose: Human TNFRSF11A/Rank Protein

Sequence: Ile30-Pro212

Characteristics: Recombinant Human TNFRSF11A/Rank Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ile30-Pro212.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human RANKL, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: TNFRSF11A

Background: TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.

Molecular Weight: 46.85 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

UniProt: Q9Y6Q6-1

Pathways: NF-kappaB Signaling