TNFRSF11A Protein (AA 31-214) (hFc Tag)

Catalog No. ABIN7275522

This Recombinant TNFRSF11A protein is produced in HEK-293 Cells.

Quick Overview for TNFRSF11A Protein (AA 31-214) (hFc Tag) (ABIN7275522)

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-214

Purification tag / Conjugate: This TNFRSF11A protein is labelled with hFc Tag.

Purpose: Mouse TNFRSF11A/Rank Protein

Sequence: Val31-Ser214

Characteristics: Recombinant Mouse TNFRSF11A/Rank Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Val31-Ser214.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Mouse RANKL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Mouse TNFRSF11A, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.,Mouse TNFRSF11A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TNFRSF11A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF11A (Tumor Necrosis Factor Receptor Superfamily, Member 11a, NFKB Activator (TNFRSF11A))

Alternative Name: TNFRSF11A

Background: TNFRSF11A, also known as receptor activator of NF-κB (RANK), activates several signaling pathways, such as NF-κB, JNK, ERK, p38α, and Akt/PKB. RANK/TNFRSF11A is a novel and frequent target for de novo methylation in gliomas, which affects apoptotic activity and focus formation thereby contributing to the molecular pathogenesis of gliomas.

Molecular Weight: 47.0 kDa. Due to glycosylation, the protein migrates to 57-67 kDa based on Bis-Tris PAGE result.

UniProt: O35305

Pathways: NF-kappaB Signaling