S100A8 Protein (AA 1-93) (His tag)

Catalog No. ABIN7275540

This Recombinant S100A8 protein is produced in Escherichia coli (E. coli).

Quick Overview for S100A8 Protein (AA 1-93) (His tag) (ABIN7275540)

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-93

Purification tag / Conjugate: This S100A8 protein is labelled with His tag.

Purpose: Human S100A8 Protein

Sequence: Met1-Glu93

Characteristics: Recombinant Human S100A8 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Glu93.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human S100A8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human S100A8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 2 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Alternative Name: S100A8

Background: S100A8 and S100A9 (also known as MRP8 and MRP14, respectively) are Ca2 binding proteins belonging to the S100 family. They often exist in the form of heterodimer, while homodimer exists very little because of the stability. S100A8/A9 is constitutively expressed in neutrophils and monocytes as a Ca2 sensor, participating in cytoskeleton rearrangement and arachidonic acid metabolism.

Molecular Weight: 11.7 kDa same as Bis-Tris PAGE result.

UniProt: P05109

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins