SARS-CoV NSP3 Protein (AA 1-314) (His-Avi Tag)

Catalog No. ABIN7275547

This Recombinant SARS-CoV NSP3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for SARS-CoV NSP3 Protein (AA 1-314) (His-Avi Tag) (ABIN7275547)

Target: SARS-CoV NSP3 (SARS-Coronavirus Nonstructural Protein 3 (SARS-CoV NSP3))

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus (SARS-CoV)

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-314

Purification tag / Conjugate: This SARS-CoV NSP3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated SARS PLpro/papain-like protease Protein

Sequence: Glu1-Ile314

Characteristics: Recombinant SARS-CoV-2 PLpro/papain-like protease Protein is expressed from E.coil with His tag and Avi tag at the N-Terminus.It contains Glu1-Ile314.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: SARS-CoV-2 PLpro on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS PLpro is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM Tris, 100 mM NaCl, 10 % Glycerol ( pH 7.8).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV NSP3 (SARS-Coronavirus Nonstructural Protein 3 (SARS-CoV NSP3))

Alternative Name: PLpro

Target Type: Viral Protein

Background: The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. Although the primary function of PLpro and 3CLpro are to process the viral polyprotein in a coordinated manner, PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses.

Molecular Weight: 38.6 kDa same as Bis-Tris PAGE result.

UniProt: P0C6U8