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SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag)

Name: SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag)
Brand: antibodies-online
SKU: ABIN7275565
Price: 364.25 USD
Availability: InStock

This Recombinant SEMA4D/CD100 protein is expressed in HEK-293 Cells.

Human Semaphorin 4D on Tris-Bis PAGE under reduced condition. (SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag))

SDS

SEC-HPLC

Immobilized Human Semaphorin 4D, His Tag at 1 μg/mL (100 μL/well) on the plate. (SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag))

ELISA

3 images

Catalog No. ABIN7275565

Quick Overview for SEMA4D/CD100 Protein (AA 22-734) (His-Avi Tag) (ABIN7275565)

Target

See all SEMA4D/CD100 (SEMA4D) Proteins

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Protein Type

Recombinant

Origin

Human

Source

HEK-293 Cells

Purity

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Discover our top product SEMA4D Protein

Protein Characteristics

AA 22-734

Purification tag / Conjugate

This SEMA4D/CD100 protein is labelled with His-Avi Tag.

Purpose

Human Semaphorin 4D/SEMA4D/CD100 Protein

Sequence

Met22-Arg734

Characteristics

Recombinant Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

Sterility

0.22 μm filtered

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity Comment

Immobilized Human Semaphorin 4D, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. See testing image for detail.
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Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His-Avi Tag,Biotin)
SEMA4D Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-734) protein (His tag)
SEMA4D Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 24-733) protein (His tag)
SEMA4D Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)
SEMA4D Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 759-862) protein (His-IF2DI Tag)
SEMA4D Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (AA 22-734) protein (His tag)
SEMA4D Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (Fc Tag)
SEMA4D Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (Active) protein (His tag)
SEMA4D Origin: Mouse Host: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) protein (His tag)
SEMA4D Origin: Rat Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE

Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D) (Active) protein (Fc Tag)
SEMA4D Origin: Human Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE. Active

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage

-20 °C,-80 °C

Storage Comment

-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date

12 months
Target

SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name

Semaphorin 4D

Background

Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Molecular Weight

82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.

UniProt

Q92854-1

Pathways

Regulation of Cell Size