SEMA4D/CD100 Protein (AA 22-734) (Biotin,His-Avi Tag)

Catalog No. ABIN7275566

Recombinant SEMA4D/CD100 protein expressed in HEK-293 Cells.

Quick Overview for SEMA4D/CD100 Protein (AA 22-734) (Biotin,His-Avi Tag) (ABIN7275566)

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-734

Purification tag / Conjugate: This SEMA4D/CD100 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein

Sequence: Met22-Arg734

Characteristics: Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.,Biotinylated Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: Semaphorin 4D

Background: Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Molecular Weight: 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

UniProt: Q92854

Pathways: Regulation of Cell Size