SEZ6 Protein (AA 20-925) (His tag)

Catalog No. ABIN7275572

Recombinant SEZ6 protein expressed in HEK-293 Cells.

Quick Overview for SEZ6 Protein (AA 20-925) (His tag) (ABIN7275572)

Target: SEZ6 (Seizure Related 6 Homolog (SEZ6))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 20-925

Purification tag / Conjugate: This SEZ6 protein is labelled with His tag.

Purpose: Human SEZ6 Protein

Sequence: Leu20-His925

Characteristics: Recombinant Human SEZ6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-His925.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SEZ6, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-SEZ6 Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SEZ6 (Seizure Related 6 Homolog (SEZ6))

Alternative Name: SEZ6

Background: Seizure-related protein 6 (Sez6) contributes to chronic pain development as sez6 knockout mice show attenuated pain behaviours after peripheral nerve injury, compared with control mice. The type I transmembrane isoform of Sez6 is cleaved by the β-amyloid precursor protein cleavage enzyme 1 (BACE1), resulting in Sez6 extracellular domain shedding from the neuron surface.

Molecular Weight: 98.9 kDa. Due to glycosylation, the protein migrates to 145-150 kDa based on Tris-Bis PAGE result.

UniProt: Q53EL9-1

Pathways: Synaptic Membrane