SFRP1 Protein (AA 32-314) (His tag)

Catalog No. ABIN7275578

Recombinant SFRP1 protein expressed in HEK-293 Cells.

Quick Overview for SFRP1 Protein (AA 32-314) (His tag) (ABIN7275578)

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-314

Purification tag / Conjugate: This SFRP1 protein is labelled with His tag.

Purpose: Human SFRP1/SARP2 Protein

Sequence: Ser32-Lys314

Characteristics: Recombinant Human SFRP1/SARP2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser32-Lys314.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human SFRP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human SFRP1 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SFRP1 (Secreted Frizzled-Related Protein 1 (SFRP1))

Alternative Name: SFRP1

Background: Secreted frizzled-related protein 1 (SFRP1) is a gene that belongs to the secreted glycoprotein SFRP family. SFRP1 has been classified as a tumor suppressor gene due to the loss of expression in various human cancers, which is mainly attributed by epigenetic inactivation via DNA methylation or transcriptional silencing by microRNAs.

Molecular Weight: 33.63 kDa. Due to glycosylation, the protein migrates to 43-48 kDa based on Bis-Tris PAGE result.

UniProt: Q8N474

Pathways: WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Negative Regulation of Hormone Secretion, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance, Tube Formation, Positive Regulation of fat Cell Differentiation