SG3 (AA 20-468) (Active) protein (His tag)

Catalog No. ABIN7275579

This Recombinant SG3 protein is produced in HEK-293 Cells.

Quick Overview for SG3 (AA 20-468) (Active) protein (His tag) (ABIN7275579)

Target: SG3

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 20-468

Purification tag / Conjugate: His tag

Purpose: Human SG3/Secretogranin 3 Protein

Sequence: Phe20-Leu468

Characteristics: Recombinant Human SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe20-Leu468.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SG3, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SG3 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.,Human SG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: SG3

Background: Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.

Molecular Weight: 52.1 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Bis-Tris PAGE result.

UniProt: Q8WXD2