SG3 (AA 23-471) (Active) protein (His tag)
Quick Overview for SG3 (AA 23-471) (Active) protein (His tag) (ABIN7275580)
Target
Protein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-471
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Purification tag / Conjugate
- His tag
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Purpose
- Mouse SG3/Secretogranin 3 Protein
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Sequence
- Phe23-Leu471
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Characteristics
- Recombinant Mouse SG3/Secretogranin 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe23-Leu471.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Mouse SG3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse SG3 is greater than 95% as determined by SEC-HPLC.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- SG3
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Background
- Secretogranin III (Scg3) is a member of the granin protein family that regulates the biogenesis of secretory granules. Scg3 was recently discovered as an angiogenic factor, expanding its functional role to extrinsic regulation. Unlike many other known angiogenic factors, the pro-angiogenic actions of Scg3 are restricted to pathological conditions. Among thousands of quantified endothelial ligands, Scg3 has the highest binding activity ratio to diabetic vs.
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Molecular Weight
- 52 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
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UniProt
- P47867
Target
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