DC-SIGN/CD209 Protein (AA 59-404) (His tag)

Catalog No. ABIN7275586

This Recombinant DC-SIGN/CD209 protein is expressed in HEK-293 Cells.

Quick Overview for DC-SIGN/CD209 Protein (AA 59-404) (His tag) (ABIN7275586)

Target: DC-SIGN/CD209 (CD209) (CD209)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 59-404

Purification tag / Conjugate: This DC-SIGN/CD209 protein is labelled with His tag.

Purpose: Human CD209/DC-SIGN Protein

Sequence: Gln59-Ala404

Characteristics: Recombinant Human CD209/DC-SIGN Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln59-Ala404.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human BTN2A1, His Tag immobilized on CM5 Chip can bind Human CD209, His Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).,Human CD209 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human CD209 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: DC-SIGN/CD209 (CD209) (CD209)

Alternative Name: CD209

Background: C-type lectin CD209/DC-SIGN and CD209L/L-SIGN proteins are distinct cell adhesion and pathogen recognition receptors that mediate cellular interactions and recognize a wide range of pathogens, including viruses such as SARS, SARS-CoV-2, bacteria, fungi and parasites. Pathogens exploit CD209 family proteins to promote infection and evade the immune recognition system.

Molecular Weight: 40.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.

UniProt: Q9NNX6