SIGLEC5 Protein (AA 17-434) (His-Avi Tag)

Catalog No. ABIN7275598

This Recombinant SIGLEC5 protein is expressed in HEK-293 Cells.

Quick Overview for SIGLEC5 Protein (AA 17-434) (His-Avi Tag) (ABIN7275598)

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 17-434

Purification tag / Conjugate: This SIGLEC5 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Siglec-5/CD170 Protein

Sequence: Glu17-Thr434

Characteristics: Recombinant Human Siglec-5/CD170 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu17-Thr434.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Siglec-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Siglec-5 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Alternative Name: Siglec-5

Background: Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Siglec 5 is putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Molecular Weight: 49.3 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result.

UniProt: O15389