SIGLEC10 Protein (AA 17-546, R119A) (His-Avi Tag)

Catalog No. ABIN7275604

This Recombinant SIGLEC10 protein is expressed in HEK-293 Cells.

Quick Overview for SIGLEC10 Protein (AA 17-546, R119A) (His-Avi Tag) (ABIN7275604)

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 17-546, R119A

Purification tag / Conjugate: This SIGLEC10 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human Siglec-10 (R119A) Protein

Sequence: Met17-Thr546 (R119A)

Characteristics: Recombinant Human Siglec-10 (R119A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met17-Thr546.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Immobilized Human Siglec-10 (R119A), His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA (QC Test).,Human Siglec-10 (R119A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM Tris,150 mM NaCl, 200 mM Arginine ( pH 8.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: Siglec-10

Background: Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.

Molecular Weight: 62.64 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Bis-Tris PAGE result.

UniProt: Q96LC7