SIGLEC5 Protein (AA 17-439) (His tag)
Quick Overview for SIGLEC5 Protein (AA 17-439) (His tag) (ABIN7275613)
Target
See all SIGLEC5 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 17-439
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Purification tag / Conjugate
- This SIGLEC5 protein is labelled with His tag.
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Purpose
- Mouse Siglec-5/CD170 Protein
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Sequence
- Thr17-Leu439
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Characteristics
- Recombinant Mouse Siglec-5/CD170 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr17-Leu439.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per ug by the LAL method.
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Biological Activity Comment
- Mouse Siglec-5/CD170 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse Siglec-5/CD170 is greater than 95% as determined by SEC-HPLC.
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Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) (Active) protein (His-Avi Tag)
SIGLEC5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) (Active) protein (Biotin,His-Avi Tag)SIGLEC5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 465-549) protein (GST tag)SIGLEC5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) protein (His tag)SIGLEC5 Origin: Cynomolgus Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) protein (His tag)SIGLEC5 Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) protein (His tag)SIGLEC5 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-434) (Active) protein (hFc Tag,Biotin)SIGLEC5 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5) (AA 17-441) protein (hIgG-His-tag)SIGLEC5 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
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Alternative Name
- Siglec-5
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Background
- Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1.
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Molecular Weight
- 46.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.
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UniProt
- Q920G3
Target
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