Sclerostin Protein (SOST) (AA 69-258) (His tag)

Catalog No. ABIN7275635

Recombinant Sclerostin protein expressed in HEK-293 Cells.

Quick Overview for Sclerostin Protein (SOST) (AA 69-258) (His tag) (ABIN7275635)

Target: Sclerostin (SOST)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 69-258

Purification tag / Conjugate: This Sclerostin protein is labelled with His tag.

Purpose: Cynomolgus SOST/Sclerostin Protein

Sequence: Gln69-Tyr258

Characteristics: Recombinant Cynomolgus SOST/Sclerostin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln69-Tyr258.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus SOST, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test).,Immobilized Cynomolgus SOST, His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 10.0ng/ml determined by ELISA.,Cynomolgus SOST on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Sclerostin (SOST)

Alternative Name: SOST

Background: SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Molecular Weight: 22.58 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005584428