SIRP AlphaV8 (AA 31-369) (Active) protein (Biotin,His-Avi Tag)

Catalog No. ABIN7275672

This Recombinant SIRP AlphaV8 protein is produced in HEK-293 Cells.

Quick Overview for SIRP AlphaV8 (AA 31-369) (Active) protein (Biotin,His-Avi Tag) (ABIN7275672)

Target: SIRP AlphaV8

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-369

Purification tag / Conjugate: Biotin,His-Avi Tag

Purpose: Biotinylated Human SIRP alpha V8 Protein

Sequence: Glu31-Arg369(S44L, S50T, I52T, H54R, V57A)

Characteristics: Recombinant Biotinylated Human SIRP alpha V8 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu31-Arg369(S44L, S50T, I52T, H54R, V57A).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human SIRP alpha V8, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).,Biotinylated Human SIRP alpha V8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human SIRP alpha V8 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: SIRP AlphaV8

Alternative Name: SIRP alpha V8

Background: Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Molecular Weight: 40.1 kDa. Due to glycosylation, the protein migrates to 52-68 kDa based on Bis-Tris PAGE result.