Syndecan 1 Protein (SDC1) (AA 23-254) (His tag)

Catalog No. ABIN7275677

Recombinant Syndecan 1 protein expressed in HEK-293 Cells.

Quick Overview for Syndecan 1 Protein (SDC1) (AA 23-254) (His tag) (ABIN7275677)

Target: Syndecan 1 (SDC1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 23-254

Purification tag / Conjugate: This Syndecan 1 protein is labelled with His tag.

Purpose: Human Syndecan-1 Protein

Sequence: Gln23-Gly254

Characteristics: Recombinant Human Syndecan-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln23-Gly254.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Syndecan-1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Syndecan-1 Antibody, hFc Tag with the EC50 of 23.2ng/ml determined by ELISA.,Human Syndecan-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: Syndecan 1 (SDC1)

Alternative Name: Syndecan-1

Background: Syndecan-1 (SDC-1) is a heparan sulfate (HS)/chondroitin sulfate proteoglycan (PG) of the cell surface and the extracellular matrix (ECM), which regulates a broad spectrum of physiological and pathological processes such as cell proliferation, migration, inflammation, matrix remodeling, wound healing, and tumorigenesis. Syndecan-1 represents the major PG of the liver, expressed by hepatocytes and cholangiocytes, and its elevated expression is a characteristic feature of liver diseases.

Molecular Weight: 25 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Bis-Tris PAGE result.

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Lipid Metabolism