TAG-72 Protein (AA 1-478) (His tag)

Catalog No. ABIN7275685

This Recombinant TAG-72 protein is expressed in Escherichia coli (E. coli).

Quick Overview for TAG-72 Protein (AA 1-478) (His tag) (ABIN7275685)

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Protein Type: Recombinant

Biological Activity: Active

Origin: Dog

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-478

Purification tag / Conjugate: This TAG-72 protein is labelled with His tag.

Purpose: Canine TAG-72 Protein

Sequence: Met1-Gln478

Characteristics: Recombinant Canine TAG-72 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Met1-Gln478.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Canine TAG-72 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Canine TAG-72 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Alternative Name: TAG-72

Background: The guanine-N7 methyltransferase domain of vaccinia virus mRNA capping enzyme is a heterodimer composed of a catalytic subunit and a stimulatory subunit. Cap (guanine-N7) methylation is an essential step in eukaryal mRNA synthesis and a potential target for antiviral, antifungal, and antiprotozoal drug discovery.

Molecular Weight: 56.19 kDa same as Bis-Tris PAGE result.

NCBI Accession: XP_005615459