CD248 Protein (AA 18-687) (His tag)

Catalog No. ABIN7275687

This Recombinant CD248 protein is produced in HEK-293 Cells.

Quick Overview for CD248 Protein (AA 18-687) (His tag) (ABIN7275687)

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 18-687

Purification tag / Conjugate: This CD248 protein is labelled with His tag.

Purpose: Human TEM1/cd248 Protein

Sequence: Gln18-Leu687

Characteristics: Recombinant Human TEM1/cd248 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln18-Leu687.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TEM1, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-TEM1 Antibody, hFc Tag with the EC50 of 2.0ng/ml determined by ELISA.,Human TEM1 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.,The purity of Human TEM1 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CD248 (CD248 Molecule, Endosialin (CD248))

Alternative Name: TEM1

Background: Colorectal cancer (CRC) is one of the most common cancers worldwide usually diagnosed in the advanced stage. The serum concentration of tumor endothelial marker 1 (TEM1) was measured and correlated with clinicopathological features to evaluate whether TEM1 might serve as a biomarker for early CRC diagnosis, progression, and prognosis. TEM1 can act as a potential diagnostic, progression, and prognostic serum biomarker for patients with CRC, TEM1 might be a good supplement for commonly used markers CEA and Ca 19-9.

Molecular Weight: 72.7 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.

UniProt: Q9HCU0