TMEM106B Protein (AA 119-275) (hFc Tag)

Catalog No. ABIN7275691

This Recombinant TMEM106B protein is expressed in HEK-293 Cells.

Quick Overview for TMEM106B Protein (AA 119-275) (hFc Tag) (ABIN7275691)

Target: TMEM106B (Transmembrane Protein 106B (TMEM106B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 119-275

Purification tag / Conjugate: This TMEM106B protein is labelled with hFc Tag.

Purpose: Mouse TMEM106B Protein

Sequence: Pro119-Gln275

Characteristics: Recombinant Mouse TMEM106B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Pro119-Gln275.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TMEM106B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TMEM106B is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TMEM106B (Transmembrane Protein 106B (TMEM106B))

Alternative Name: TMEM106B

Background: TMEM106B is a well-recognised risk factor for FTD caused by GRN mutation. Elegant experiments have suggested that increased risk for FTD is due to elevated levels of TMEM106B (Nicholson et al, 2013, Gallagher et al, 2017). Therefore, recent work has explored the therapeutic potential of reducing TMEM106B levels, with initial results looking encouraging, as crossing a Grn-deficient mouse to a Tmem106b knockout showed a rescue in FTD-related behavioural defects and specific aspects of lysosome dysfunction (Klein et al, 2017).

Molecular Weight: 44.8 kDa. Due to glycosylation, the protein migrates to 65-68 kDa based on Bis-Tris PAGE result.

UniProt: Q80X71