TFF1 Protein (AA 22-87) (hFc Tag)

Catalog No. ABIN7275693

This Recombinant TFF1 protein is produced in HEK-293 Cells.

Quick Overview for TFF1 Protein (AA 22-87) (hFc Tag) (ABIN7275693)

Target: TFF1 (Trefoil Factor 1 (TFF1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-87

Purification tag / Conjugate: This TFF1 protein is labelled with hFc Tag.

Purpose: Mouse TFF1 Protein

Sequence: Gln22-Phe87

Characteristics: Recombinant Mouse TFF1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln22-Phe87.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse TFF1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TFF1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TFF1 (Trefoil Factor 1 (TFF1))

Alternative Name: TFF1

Background: Breast cancer (BC) is the most common cancer in women and the second leading cause of their cancer death. Establishing an accurate BC prognosis is very difficult because of its heterogeneity. Elevated TFF1 levels in serum were associated with development of BC, TFF1 expression was upregulated in BC compared to the healthy breast tissue.That expression of TFF1 was related to ER status of BC and that expression of TFF1 was lower in TNBC than in non-TNBC.

Molecular Weight: 34 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Bis-Tris PAGE result.

UniProt: Q08423

Pathways: EGFR Signaling Pathway