TFPI Protein (AA 29-282) (Biotin,His-Avi Tag)
Quick Overview for TFPI Protein (AA 29-282) (Biotin,His-Avi Tag) (ABIN7275697)
Target
See all TFPI ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 29-282
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Purification tag / Conjugate
- This TFPI protein is labelled with Biotin,His-Avi Tag.
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Purpose
- Biotinylated Human TFPI Protein
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Sequence
- Asp29-Lys282
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Characteristics
- Recombinant Biotinylated Human TFPI Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp29-Lys282.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Biotinylated Human TFPI on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, the IC50 value is < 0.75 nM.
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Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-282) (Active) protein (His-Avi Tag)
TFPI Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 152-251) protein (GST tag)TFPI Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-251) protein (GST tag)TFPI Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-282) (Active) protein (His tag)TFPI Origin: Human Host: Mammalian Cells Recombinant >90 % as determined by SDS-PAGE. SDS, WB, Imm, ELISA, AcA Active
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (Active) protein (His tag)TFPI Origin: Mouse Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-304) protein (His tag)TFPI Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % SDS
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-304) protein (His tag)TFPI Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % SDS
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-280), (partial) protein (GST tag)TFPI Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
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Alternative Name
- TFPI
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Background
- Oestrogens influence the pathophysiology and development of hormone-sensitive cancers, such as breast cancer. Tissue factor pathway inhibitor (TFPI) is a serine protease inhibitor of the extrinsic coagulation pathway and has recently been associated with breast cancer cell development.
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Molecular Weight
- 32.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
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UniProt
- P10646
Target
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