Transferrin Receptor Protein (AA 89-760) (Biotin,His-Avi Tag)

Catalog No. ABIN7275703

This Recombinant Transferrin Receptor protein is expressed in HEK-293 Cells.

Quick Overview for Transferrin Receptor Protein (AA 89-760) (Biotin,His-Avi Tag) (ABIN7275703)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 89-760

Purification tag / Conjugate: This Transferrin Receptor protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Transferrin R/CD71 Protein

Sequence: Cys89-Phe760

Characteristics: Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Transferrin, His Tag immobilized on CM5 Chip can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 7.73 nM as determined in SPR assay (Biacore T200).,Immobilized Human Transferrin, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 66.4ng/ml determined by ELISA (QC Test).,Immobilized Biotinylated Human Transferrin R, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Transferrin R Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA.,Biotinylated Human Transferrin R on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.,Loaded Anti-Transferrin R Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human Transferrin R, His-Avi Tag with an affinity constant of 0.24 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 200 mM L-arginine, 8 % trehalose ( pH 7.4).

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: Transferrin R

Background: The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Molecular Weight: 77.9 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis