TGFB2 Protein (AA 303-414) (AVI tag,Biotin)

Catalog No. ABIN7275711

This Recombinant TGFB2 protein is produced in HEK-293 Cells.

Quick Overview for TGFB2 Protein (AA 303-414) (AVI tag,Biotin) (ABIN7275711)

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 303-414

Purification tag / Conjugate: This TGFB2 protein is labelled with AVI tag,Biotin.

Purpose: Biotinylated Human Mature TGF beta 2 Protein

Sequence: Ala303-Ser414

Characteristics: Recombinant Biotinylated Human Mature TGF beta 2 Protein is expressed from HEK293 with Avi tag at the C-Terminus.It contains Ala303-Ser414.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).,Biotinylated Human Mature TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFB2 (Transforming Growth Factor, beta 2 (TGFB2))

Alternative Name: TGF beta 2

Background: Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.

Molecular Weight: 14.53 kDa same as Bis-Tris PAGE result.

UniProt: P61812

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus