TGFB3 Protein (AA 301-412)

Catalog No. ABIN7275712

This Recombinant TGFB3 protein is expressed in HEK-293 Cells.

Quick Overview for TGFB3 Protein (AA 301-412) (ABIN7275712)

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 301-412

Purpose: Human Mature TGF beta 3 Protein

Sequence: Ala301-Ser412

Characteristics: Recombinant Human Mature TGF beta 3 Protein is expressed from HEK293 without tag.It contains Ala301-Ser412.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human Mature TGF beta 3, No Tag at 1ug (100ul/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA (QC Test).,Human Mature TGF beta 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 10-80 pg/ml (QC Test).,Loaded Human TGF-beta RII, hFc Tag on ProA-Biosensor can bind Human Mature TGF beta 3, No Tag with an affinity constant of 0.51 nM as determined in BLI assay (Gator® Prime).

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 4 mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 4 mM HCl. Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Alternative Name: TGF beta 3

Background: Transforming growth factor-beta 3 is a cytokine which is involved in cell growth regulation and differentiation, stimulation of extracellular matrix and modulation of immune responses. TGF beta 3 RNA is expressed in a diverse array of tissues including perichondrium, bone, intervertebral discs, mesenteries, pleura, heart, lung, palate, and amnion, as well as in central nervous system (CNS) structures such as the meninges, choroid plexus and the olfactory bulbs. Furthermore, in several organ systems, TGF beta 3 transcripts are expressed during periods of active morphogenesis suggesting that the protein may be an important factor for the growth and differentiation of many embryonic tissues.

Molecular Weight: 12.7 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

UniProt: P10600

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus