TGFBR2 Protein (AA 24-159) (hFc Tag)

Catalog No. ABIN7275719

Recombinant TGFBR2 protein expressed in HEK-293 Cells.

Quick Overview for TGFBR2 Protein (AA 24-159) (hFc Tag) (ABIN7275719)

Target: TGFBR2 (Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-159

Purification tag / Conjugate: This TGFBR2 protein is labelled with hFc Tag.

Purpose: Human TGF-beta RII/TGFBR2 Protein

Sequence: Ile24-Asp159

Characteristics: Recombinant Human TGF-beta RII/TGFBR2 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Ile24-Asp159.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 uM as determined in SPR assay (Biacore T200).,Immobilized Human Mature TGF beta 1, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).,Human TGF-beta RII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human TGF-beta RII, hFc Tag on ProA-Biosensor can bind Human Mature TGF beta 3, No Tag with an affinity constant of 0.51 nM as determined in BLI assay (Gator® Prime).,The purity of Human TGF-beta RII is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: TGFBR2 (Transforming Growth Factor, beta Receptor II (70/80kDa) (TGFBR2))

Alternative Name: TGF-beta RII

Background: The TGFBR2 gene provides instructions for making a protein called transforming growth factor-beta (TGF-β) receptor type 2. This receptor transmits signals from the cell surface into the cell through a process called signal transduction. Through this type of signaling, the environment outside the cell affects activities inside the cell such as stimulation of cell growth and division.

Molecular Weight: 44.2 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Bis-Tris PAGE result.

UniProt: P37173