Transglutaminase 2 Protein (AA 2-686) (His tag)

Catalog No. ABIN7275724

This Recombinant Transglutaminase 2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for Transglutaminase 2 Protein (AA 2-686) (His tag) (ABIN7275724)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 2-686

Purification tag / Conjugate: This Transglutaminase 2 protein is labelled with His tag.

Purpose: Mouse TGM2 Protein

Sequence: Ala2-Ala686

Characteristics: Recombinant Mouse TGM2 Protein is expressed from E.coli with His tag at the N-Terminus.It contains Ala2-Ala686.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per ug by the LAL method.

Biological Activity Comment: Mouse TGM2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse TGM2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: TGM2

Background: Transglutaminase 2 (TGM2) is a member of the transglutaminase family, and it is reported to be associated with chemoresistance in various types of cancer. TGM2 was demonstrated to affect the chemosensitivity of osteosarcoma cells via regulation of the activation of mitogen-activated protein kinase and AKT serine/threonine kinase pathways.

Molecular Weight: 78.2 kDa same as Bis-Tris PAGE result.

UniProt: P21981

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling