TIGIT Protein (AA 22-141) (Biotin,His-Avi Tag)

Catalog No. ABIN7275730

This Recombinant TIGIT protein is expressed in HEK-293 Cells.

Quick Overview for TIGIT Protein (AA 22-141) (Biotin,His-Avi Tag) (ABIN7275730)

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-141

Purification tag / Conjugate: This TIGIT protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human TIGIT Protein

Sequence: Met22-Pro141

Characteristics: Recombinant Biotinylated Human TIGIT Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Pro141.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.,Biotinylated Human TIGIT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Alternative Name: TIGIT

Background: TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.

Molecular Weight: 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Bis-Tris PAGE result.

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints